ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (11): 1784-1792.doi: 10.11843/j.issn.0366-6964.2014.11.007

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Detection of DNA Methylation in Whole Genomes of Muscle and Ovary Tissues of Beijing You Chicken

LI Jin-long1#,TANG Shao-qing2#,ZHAO Meng1,WANG Hai-chao1,XU Qing1*   

  1. (1.Institute of Life Science and Biotechnology,Beijing Jiaotong University,Beijing 100044,China;2.Beijing Animal Husbandry Station,Beijing 100029,China)
  • Received:2014-03-27 Online:2014-11-23 Published:2014-11-23

Abstract:

 The DNA methylation extent and pattern was assessed in genomes in muscle and ovary tissues from the 17-week-old Beijing You chicken using a methylation sensitive amplified polymorphism (MSAP) approach in this study.We analyzed different DNA methylation levels in different tissues.A total of 4 297 clear bands were obtained using 12 pairs of selective amplification primers,about 10-20 bands per sample with a pair of primers.The degree of methylation was approximate 35.86% in muscle and 31.50% in ovary in Beijing You chickens,and a significant difference was found between 2 tissues at the methylation levels (P<0.01).Further analysis indicated that no significant relationship was detected in ovary tissue between the methylation levels and individual weight (P>0.05),but strongly negative correlation was depicted in muscle tissue (P<0.01).Five tissue-specific MSAP fragments were isolated and verified.Overall results reveal that there is a significant difference at the methylation levels between muscle and ovary tissues,and the methylated state of muscle tissue may play an important role in the body weight of Beijing You chicken.

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